Hi Dionne, interesting study you showed here (a topic that we are exploring in Belgium as well). I was wondering what you specifically measured at the different body sites, as you main outcome was muscle mass? However, based on the pictures you showed, I would suppose your are measuring adipose tissue thickness?
Hi Kenneth, thank you for your question. We are indeed measuring the subcutaneous adipose tissue thickness at the different body sites. Using this data and the muscle sound algorithm, we can calculate both total body adipose tissue and lean mass.
Is there any reference available for the muscle sound algorithm or it this algorithm only being set up?
Second, do you have any idea about the applicability of adipose tissue/muscle ultrasound measurements in obese children/adolescents?
Third, are the measurements being standardized (position, nutrional status, time of the day) between the different visits? For our measurements, we use the protocol of Müller, W. et al. (2016). Br J Sports Med, 50(1), 45-54.
The algorithm is based on the seven-site Jackson-Pollack prediction equations (originally developed in skinfold tissue measurements).
The algorithm is based on adults, and has only been validated in healthy/athletic populations and critically ill populations. I personally think that the ultrasound could be a good tool to look at body composition in children and adolescents with obesity, although a different algorithm should be used in this population.
Lastly, the measurements were indeed standardized. The position of the measurement was measured out using a measuring tape (and done on the same side of the body) and the time of day was similar between the measurements. For this particular study, the measurements were done one day apart, so the nutritional status was not a problem in this study.