what is in the lipid mix (e.g. FA profile, phospholipids, other lipids)?
We used a commercially available lipid mixture from sigma, which was comprised fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic) and cholesterol.
Nice work Imogen! Do you also know what the effects of the lipid mix are directly on the iNKT cells?
Indeed here we have focused on the lipid loading of adipose and its effect on subsequent crosstalk with iNKT’s. However, of course it goes both ways, and considering that iNKT’s are substantially diminished in obesity, I would suspect a longer incubation with lipid mixture, as we did for the adipose, would have the same outcome.
Thanks! Do you know if the effect is also reversible? So kind of starving the adipocytes after treatment with the lipid mix until they reach TG levels of the non-treated adipocytes followed by exposure to the iNKT cells?